Table 1. Primer Sequencesa.
| target gene | forward primer | reverse primer |
|---|---|---|
| COL1A1 | TCCAACGAGATCGAGATCC | AAGCCGAATTCCTGGTCT |
| COL3A1 | GATCCGTTCTCTGCGATGAC | AGTTCTGAGGACCAGTAGGG |
| ELN | TTTGGCCCGGGAGTAGTTGG | CAGCTGCTTCTGGTGACACAAC |
| MMP2 | TCCAAGTCTGGAGCGATGTG | CCGTCCTTACCGTCAAAGGG |
| MMP9 | GAGGTGGACCGGATGTTCC | AACTCACGCGCCAGTAGAAG |
| ACTA2 | CCTGACTGAGCGTGGCTATT | GATGAAGGATGGCTGGAACA |
| HPRT1 | CTCAACTTTAACTGGAAAGAATGTC | TCCTTTTCACCAGCAAGCT |
| YWHAZ | GATGAAGCCATTGCTGAACTTG | CTATTTGTGGGACAGCATGGA |
a
Primer sequences for the target genes of collagen I and III, elastin, MMP-2, and α-SMA. Primer sequences for housekeeping genes HPRT1 and Ywhaz. COL1A1, α1(I)procollagen; COL3A1, α1(III)procollagen; ELN, elastin; HPRT, hypoxanthine phoshoribosyltransferase; YWHAZ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.