TABLE 1.
Frequency of leukocyte accumulation within blood vesselsa
| C. perfringens strain | Total no. of blood vessels | No. (%) of vessels with:
|
|
|---|---|---|---|
| Moderate leukocyte accumulation | Severe leukocyte accumulation | ||
| Wild-type heat-killed (JIR325)c | 201 | 9 (4.5) | 0 |
| Wild-type (JIR325)b | 478 | 46 (9.6) | 41 (8.6) |
| Theta-toxin-deficient (JIR4081)c | 485 | 27 (5.6) | 3 (0.6) |
| Alpha-toxin-deficient (JIR4120)bc | 466 | 72 (15.5) | 14 (3.0) |
a
Analysis of the frequency of leukocyte accumulation within blood vessels of tissue samples taken 8 to 12 h postinjection. Moderate leukocyte accumulation indicates that the vessel had a single layer of leukocytes lining the luminal surface of the blood vessel (an example of such a vessel is indicated in Fig. 1I). Severe leukocyte accumulation indicates multiple leukocyte layers (an example of such a vessel is indicated in Fig. 1E).
b
Differs significantly from tissues injected with heat-killed C. perfringens cells (P < 0.0001).
c
Differs significantly from tissues injected with wild-type C. perfringens cells (P < 0.0001).