Table 2. Effect of Washing on the Distribution of APAP, CAF, COL, and FLUa.
| test chemical [CNom] | incubation time [h] | total CMedium[μmol/L medium] | total CPBS1[μmol/L PBS] | total CPBS2[μmol/L PBS] | total CCell[μmol/L cell] |
|---|---|---|---|---|---|
| APAP [60 μmol/L medium] | 0 | 67.8 ± 1.9 (100%) | |||
| 6 | 50.0 ± 10.3 (74%) | 2.6 ± 0.9 (3.8%) | <0.8 (<1.1%) | 75.1 ± 33.4 (0.04%) | |
| 24 | 40.5 ± 2.7 (60%) | 2.5 ± 0.8 (3.6%) | <0.8 (<1.1%) | 120.1 ± 40.0 (0.12%) | |
| 48 | 39.1 ± 1.4 (58%) | 1.7 ± 0.4 (2.6%) | <0.8 (<1.1%) | 38.1 ± 8.0 (0.09%) | |
| CAF [160 μmol/L medium] | 0 | 160.5 ± 5.6 (100%) | |||
| 6 | 127.0 ± 15.1 (79%) | 6.7 ± 0.3 (4.2%) | 1.6 ± 0.4 (1.0%) | 182.0 ± 91.0 (0.04%) | |
| 24 | 133.1 ± 12.4 (83%) | 4.5 ± 0.7 (2.8%) | <0.9 (<0.5%) | 161.1 ± 19.3 (0.03%) | |
| 48 | 125.9 ± 15.6 (78%) | 5.1 ± 0.5 (3.2%) | <0.9 (<0.5%) | 40.9 ± 7.1 (0.03%) | |
| COL [10 μmol/L medium] | 0 | 6.5 ± 0.4 (65%) | |||
| 6 | 4.2 ± 0.5 (80%) | 0.3 ± 0.1 (4.5%) | <0.1 (< 2.3%) | 9.1 ± 3.6 (0.05%) | |
| 24 | 5.2 ± 0.7 (80%) | 0.1 ± 0.1 (0.5%) | <0.1 (< 2.3%) | 10.1 ± 1.4 (0.10%) | |
| 48 | 4.9 ± 0.2 (74%) | 0.3 ± 0.1 (4.3%) | <0.1 (< 2.3%) | 4.3 ± 0.4 (0.11%) | |
| FLU [10 μmol/L medium] | 0 | 9.0 ± 0.3 (100%) | |||
| 6 | 7.7 ± 1.4 (86%) | 0.5 ± 0.1 (5.1%) | 1.6 (0.4) (1.5%) | 250.4 ± 16.7 (0.93%) | |
| 24 | 6.4 ± 1.2 (72%) | 0.3 ± 0.1 (3.8%) | <0.1 (0.3%) | 333.1 ± 12.0 (2.5%) | |
| 48 | 5.3 ± 0.5 (59%) | 0.3 ± 0.1 (3.8%) | <0.1 (0.3%) | 344.5 ± 2.0 (6.4%) |
a
Total concentrations in the culture medium (Total CMedium) in PBS collected after the first and second washing steps (CPBS1 and CPBS2) and in cells (CCell) after 6, 24, and 48 h incubation with APAP, CAF, COL, and FLU. Data are represented as mean in μmol/L ± SD, n = 3. Values in brackets are the mass balance percentages given as mean ± SD in % (total CCell value is without conversion to cell volume).