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. 2022 Oct 24;18(16):6163–6175. doi: 10.7150/ijbs.78354

Figure 3.

Figure 3

ADCK2 depletion suppresses NSCLC cell survival, proliferation and cell motility. Puromycin-selected pCan-1 cells, with the lentiviral ADCK2 shRNA (“ADCK2-sh”), the CRISPR/Cas9-ADCK2-KO construct (“ADCK2-ko”) or the lentiviral scramble non-sense control shRNA plus the CRISPR/Cas9 control empty construct (“shScr+koC”), were formed, expression of ADCK2 mRNA (A) and protein (B) was shown; After culturing for designated time, cell viability (C), formed colonies (D), Trypan blue-positive staining cell ratio (E) and EdU-stained nuclei ratio (F), as well as cell cycle distribution (G) and in vitro cell migration (H) were tested. “pCan2” and “pCan3” primary cells, the established A549 cells, or “pEpi” lung epithelial cells, with “ADCK2-sh” or “shScr”, were established, and the expression of ADCK2 mRNA was shown (I and M). After culturing for designated time, cell viability (J and N), EdU-stained nuclei ratio (K and O) and in vitro cell migration (L) were measured, with the results always quantified. “Pare” stands for parental control cells (same for all Figures). * P < 0.05 vs. “Pare” or “shScr”. Scale bar = 100 µm.