Skip to main content
. 2022 Oct 24;18(16):6163–6175. doi: 10.7150/ijbs.78354

Figure 4.

Figure 4

ADCK2 depletion activates apoptosis in NSCLC cells. “ADCK2-sh”, “ADCK2-ko” or “shScr+koC” pCan-1 cells were cultured for applied time periods; The relative activities of caspase-3 (A) and caspase-9 (B), apoptosis-related proteins expression (C) and Histone DNA contents (D) were tested. Cell apoptosis was examined by calculating TUNEL-stained nuclei ratio (E). The stable pCan-1 primary NSCLC cells, co-treated with z-DEVD-cho (40 μM), z-VAD-cho (40 μM) or the vehicle control (0.1% DMSO), were stably infected with ADCK2-sh or ADCK2-ko, after 96h cell viability and death were respectively tested by CCK-8 (F) and Trypan blue staining (G) assays. “pCan2” and “pCan3” primary cells, A549 cells, or “pEpi” lung epithelial cells, with “ADCK2-sh” or “shScr”, were established and were cultured. The caspase-3 activity (H) and nuclear TUNEL percentage (I and J) were measured. * P < 0.05 vs. “Pare”/“shSCR” cells. # P < 0.05 vs. “DMSO” group (F and G). Scale bar = 100 µm.