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. 2022 Oct 24;18(16):6163–6175. doi: 10.7150/ijbs.78354

Figure 6.

Figure 6

Ectopic overexpression of ADCK2 exerts pro-cancerous activity in NSCLC cells. Stable “pCan-1” cells, with the ADCK2-expressing lentiviral construct (oe-ADCK2-StL1 and oe-ADCK2-StL2, two stable selections) or the GV248 empty vector (“Vec”), were established, expression of ADCK2 mRNA (A) and protein (B) was shown; After culturing for designated time, ATP contents (C), EdU-stained nuclei ratio (D), and migrated cell number (E) were measured and recorded. The pCan-1 primary NSCLC cells were infected with ADCK2 shRNA lentivirus (“ADCK2-sh”), or together with the ADCK2-expressing construct lentivirus (“+oe-ADCK2”), stable cells were formed, and ADCK2 expression was tested (F and G). After culturing for indicated time, mitochondrial complex I activity (H) and ATP contents (I) were measured. “pCan2” and “pCan3” primary cells, A549 cells, or “pEpi” lung epithelial cells with “oe-ADCK2” or “Vec” were established, expression of ADCK2 mRNA was shown (I and K); The viable cell number was recorded after 96h (J); Cell viability (L) and proliferation (M) of pEpi cells were measured. * P < 0.05 vs. “Vec” cells. Scale bar = 100 µm.