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. 2022 Oct 18;18(16):6114–6128. doi: 10.7150/ijbs.74924

Figure 3.

Figure 3

HIF-1α is necessary for the accumulation of LDs in fibroblasts. (A) The protein expression of HIF-1α in NIH 3T3 and MEF cells under hypoxia treatment (1% O2; N = 3). (B) The BODIPY staining of LDs in fibroblasts (NIH 3T3 and MEF cells) which were treated with hypoxia (1% O2) for 12h (N = 3). (C) The protein expressions of HIF-1α in NIH 3T3 and MEF cells treated with TGF-β1 (2 ng/mL) and LLC conditioned medium (LLC-CM). (D) The BODIPY staining of the LDs in the fibroblasts (NIH 3T3 and MEF cells) which were treated with TGF-β1 and LLC-CM (N = 3). (E) The protein expressions of HIF-1α were detected in HIF-1α stably knocked out (KO) fibroblasts (NIH 3T3 and MEF cells; N = 3). (F) The lipid-tox red staining of the LDs in the HIF-1α MOCK cells (MOCK) and HIF-1α KO cells (KO) (NIH 3T3 and MEF cells; Scale bar: 50 µm; N = 3). Data are shown as the Mean±SD; *p < 0.05, **p < 0.01, ***p < 0.001.