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. 2022 Nov 22;27:101. doi: 10.1186/s11658-022-00404-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Destroyed integrity of the blood–testis barrier in mice with Alkbh5 knockdown in Sertoli cells mediated by AAV8 infection. a Images showing GFP fluorescence in the testes infected with AAV8-scramble-GFP. b Immunofluorescence analysis to confirm the specificity of AAV8 infection in Sertoli cells (n = 3). DDX4, marker for germ cells but nonspermatid; SOX9, Sertoli cell marker; PNA, spermatid marker. c BTB assay detecting the integrity of the blood–testis barrier affected by AAV-mediated Alkbh5 knockdown specifically in mouse Sertoli cells