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. 2022 Nov 22;27:101. doi: 10.1186/s11658-022-00404-x

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Fig. 4 — Identification of Cdh2 mRNA as a substrate of ALKBH5 in the TM4 cell line. a Bioinformatic analysis for distribution of m⁶A peaks along the whole transcript. b Bioinformatic analysis for changes in the m⁶A enrichment score. c Biological process analysis in Gene Ontology of the genes encoded by the hypermethylated or hypomethylated RNAs. d The intergrative Genomics Viewer (IGV) plots showing the m⁶A peak (marked by red dashed box) of Cdh2 mRNA in TM4 cells transfected with scramble siRNA (SC) or Alkbh5-targeting siRNAs (siAlkbh5). e Results of m⁶A-IP qPCR showing the relative m⁶A level of Cdh2 mRNA. f Results of RIP–qPCR detecting the interaction between ALKBH5 protein and Cdh2 mRNA. Western blot analysis of the immunoprecipitated proteins is shown in the right panel. *P < 0.05