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. 2022 Oct 26;13(45):13524–13540. doi: 10.1039/d2sc04135a

Loop-swapped chimeras made with the WPD-loop of YopH incorporated into PTP1Ba.

Enzyme WPD-loop sequence Solubility Activity
graphic file with name d2sc04135a-u1.jpg graphic file with name d2sc04135a-u2.jpg Soluble Active
graphic file with name d2sc04135a-u3.jpg graphic file with name d2sc04135a-u4.jpg Soluble Active
Chimera 0 graphic file with name d2sc04135a-u5.jpg Insoluble —
Chimera 1 graphic file with name d2sc04135a-u6.jpg Soluble Inactive
Chimera 2 graphic file with name d2sc04135a-u7.jpg Insoluble —
graphic file with name d2sc04135a-u8.jpg graphic file with name d2sc04135a-u9.jpg Soluble Active
graphic file with name d2sc04135a-u10.jpg graphic file with name d2sc04135a-u11.jpg Soluble Active
Chimera 5 graphic file with name d2sc04135a-u12.jpg Insoluble —
Chimera 6 graphic file with name d2sc04135a-u13.jpg Insoluble —
graphic file with name d2sc04135a-u14.jpg graphic file with name d2sc04135a-u15.jpg Soluble Active
a

PTP1B residues are shown in red, YopH residues are shown in blue. Residues common to both are shown in black. Chimeras studied in detail in this work are underlined in column 1. The designation “—” in the activity column indicates an insoluble chimera for which activity could not be assessed.