Kinetic data for native PTPs and chimeras with pNPP at 25 °C^a.

Enzyme	pH optimum	k lim (s−1)	K M (mM)	k cat/KlimM (mM−1 s−1)	Reference
PTP1B	5.5	24.4 ± 0.4	0.58 ± 0.01	37.7 ± 1.9	23
YopH	5.5	750 ± 25	0.98 ± 0.11	735	48
YopH Chimera 3	5.5	5.10	5.69	0.90	48
Chimera 3	6.0	1.8 ± 0.1	6.9 ± 2.0	0.26 ± 0.02	This work
Chimera 4	5.5	15.4 ± 0.8	8.7 ± 3.2	5.54 ± 0.77	This work
Chimera 7	5.5	2.5 ± 0.4	0.39 ± 0.02	11.4 ± 4.0	This work

^aThe k_cat and k_cat/K_M numbers are the limiting values obtained from eqn (1) and (2). K_M values are obtained at the pH optimum for each enzyme.