Kinetic isotope effects the active chimeras together with those of native YopH and PTP1Ba.
| Enzyme | 15(V/K) | 18(V/K)bridge | Reference |
|---|---|---|---|
| PTP1B | 1.0004(2) | 1.0121(9) | 23 |
| YopH | 0.9999(3) | 1.0152(6) | 24 |
| Range of WT PTPs | 0.9999–1.0013 | 1.0118–1.0152 | 84 |
| D to N mutants | 1.0024–1.0030 | 1.0275–1.0297 | 84 |
| Chimera 3 | 1.0001(5) | 1.0138(8) | This work |
| Chimera 4 | 1.0011(6) | 1.0129(25) | This work |
| Chimera 7 | 1.0004(1) | 1.0146(5) | This work |
a
The range of KIEs is provided for a broader set of enzymes in the PTP superfamily including dual-specificity phosphatases; the specific enzymes are given in the reference indicated. The values in parenthesis are the standard errors in the last decimal place. The D to N mutant entries show how the isotope effects are affected when general acid catalysis is lost.