| EDGE-purified C-tailed DNA, ~150 ng | 7.4 μL |
| 2× KAPA HiFI HotStart ReadyMix | 25 μL |
| 10 μM primer olj376 (Ultramer) | 3 μL |
| 10 μM primer T22-87_Left | 1 μL |
| 100× SYBR green | 0.25 μL |
| PCR-grade sterile water | 13.35 μL |
| Total reaction volume | 50 μL |
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