TABLE 1.
Properties of recombinant rSA11 strains expressing NoV capsid proteins
Virus strain |
Genome segment 7 |
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Abbreviated virus name | Formal virus namea | Genome size/increase over wt (bp) | RNA (bp) | Protein product size |
NCBI accession no. | |||
Uncleaved (aa) | 2A cleaved (aa) | Uncleaved (kDa) | 2A cleaved (kDa) | |||||
rSA11-wt | RVA/Simian-lab/USA/SA11wt/2019/G3P[2] | 18,559/0 | 1,105 | 315 | NDb | 36.4 | ND | LC178572 |
rSA11-fP2 | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:fP2)/2019/G3P[2] | 19,126/567 | 1,672 | 504 | 336 + 168 | 57.1 | 38.5 + 18.6 | MN190002 |
rSA11-fP | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:fP)/2019/G3P[2] | 19,642/1,083 | 2,188 | 676 | 336 + 340 | 76.1 | 38.5 + 37.7 | MN190003 |
rSA11-fVP1 | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:fVP1)/2019/G3P[2] | 20,314/1,755 | 2,860 | 900 | 336 + 564 | 100.3 | 38.5 + 61.9 | MN190004 |
rSA11-VP1f | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:VP1f)/2019/G3P[2] | 20.272/1,713 | 2,818 | 886 | 336 + 550 | 98.6 | 38.5 + 60.1 | MN201548 |
rSA11-Ph | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:P His)/2019/G3P[2] | 19,594/1,035 | 2,140 | 660 | 336 + 324 | 74.2 | 38.5 + 35.8 | MN201549 |
rSA11-VP1th | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:VP1ThHis)/2019/G3P[2] | 20,278/1,719 | 2,824 | 888 | 336 + 552 | 98.8 | 38.5 + 60.4 | MN201547 |
rSA11-VP1h | RVA/Simian-lab/USA/SA11(NSP3-2A-NoV:VP1 His)/2019/G3P[2] | 20,260/1,701 | 2,806 | 882 | 336 + 546 | 98.2 | 38.5 + 59.8 | MZ562305 |
rSA11(RIX-NSP3)-Ph | RVA/Simian-lab/USA/SA11(RIX NSP3-2A-NoV:P His)/2021/G3P[2] | 19,563/1,004 | 2,109 | 655 | 331 + 324 | 74.2 | 38 + 35.8 | MZ643978 |
Formal strain names were assigned according to the classification of Matthijnssens et al. (76).
ND, not determined (no 2A cleavage site was present).