FIG 3.

METTL3 directly binds HCV RNA in a WTAP-dependent manner. Huh7 cells were treated with the indicated siRNA and infected with HCV (72 h, MOI 1), followed by UV-CLIP with anti-METTL3 or nonspecific IgG. (A) Immunoblot analysis of input and immunoprecipitated UV-CLIP lysates. (B) Enrichment of indicated amplicons by METTL3 or IgG immunoprecipitation, relative to input, as measured by RT-qPCR. Graph shows mean ± SD; n = 3 biological replicates. Blot is representative of 3 independent experiments. Data were analyzed by two-way ANOVA with Šidák’s multiple-comparison test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant).