FIG 5.

WTAP regulation of HCV RNA m⁶A modification is independent of its nuclear localization. (A) Relative meRIP enrichment of indicated amplicons in HCV-infected (72 h, MOI of 1) parental Huh7-, wild-type (WT)-WTAP-HA-, or WTAPΔNLS-HA-overexpressing cells. (B) Confocal micrographs of Huh7 cells overexpressing WT-WTAP-HA or WTAPΔNLS-HA stained as indicated. Graph show mean ± SD; n = 3 biological replicates for panel A with micrographs of localization shown in panel B. Scale bars = 10 μM. Data were analyzed by two-way ANOVA with Šidák’s multiple-comparison test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).