FIG 6.

WTAP regulation of HCV virion production requires METTL3 interaction but not nuclear localization. (A) Immunoblot analysis of anti-FLAG-immunoprecipitated lysates from Huh7 cells cotransfected with HA-METTL3 and indicated WTAP-FLAG constructs, either wild-type, ΔNLS, or ΔMETTL3 interaction. (B to E) Focus-forming assay of supernatant harvested from HCV-infected (48 h, MOI of 0.3) Huh7 cells overexpressing the indicated protein (B), as well as immunoblot analysis of lysates (C) and quantification of NS5A and WTAP levels and relative total protein (D and E). Graphs show mean ± SD; n = 3 biological replicates. Blots are representative of 3 independent experiments. Data were analyzed by one-way ANOVA with Šidák’s multiple-comparison test (**, P < 0.01; ***, P < 0.001).