TABLE 1.
Primer sequences for RT-qPCR
| Amplicon | Sense oligonucleotide (5′–3′) | Antisense oligonucleotide (5′–3′) |
|---|---|---|
| HCV-1 qPCR | AGAGCCATAGTGGTCTGCGG | CTTTCGCAACCCAACGCTAC |
| HCV-2 qPCR | GATAGGGTGCTTGCGAGTGCCC | GTCTTCTGGGCGACGGTTGGTG |
| HCV-3 qPCR | CATCCCCAAAGATCGGCGCTCC | CCAGCCGAGTCCCTCATTCCCA |
| HCV-4 qPCR | ATCTACCCTGGCACCATCAC | AGTAGGCCAAGCCGAACAT |
| HCV-5 qPCR | GGCTTTCTCGCGGCCTTGTTCT | CCCCACCCTATCCGGAAAGCCT |
| HCV-6 qPCR | TGACTTCAACGCCAGCACGGAC | CACTTTGGTGTGAGCCAGGGCC |
| HCV-7 qPCR | TGCTCATCTTGTTGGGCCAGGC | CTGATGTGCCAAGCTGCCACGA |
| GOLGA3 qPCR | CGTGAGACCCGAAGAACAAAAC | CACCAAAGAGGCTGTACAGTGA |
| pLEX FLAG-GFP | GCGGCCGCTATGGTGAGCAAGGGCGAGG | ACTCGAGTTAACCTTGTACAGCTCGTCCATGCC |
| pLEX WTAP-HA | GAGGATCCATGACCAACGAAGAACCTCTTCCC | GACTCGAGTTAGTAGTCTGGGACGTCGTATGGG |
| pLEX FLAG-METTL3 | CACAGATCTACCATGGATTATAAGGATGATGATG | CACGTCGACTTAAACCTATAAATTCTTAGGTTTAGAG |
| pLEX WTAPΔNLS-HA | CCGACTCTACTAGAGGATCCGCCACCATGAGTGAAACAGACTTCAAAGTTATGGC | GACTCGAGTTAGTAGTCTGGGACGTCGTATGGG |
| pEFtak-WTAP-FLAG | GGTACCATGACCAACGAAGAACCTCTT | TTATAATCAGCGGCCGCCAAAACTGAACCCTGTACATTTACAC |
| pEFtak-HA-METTL3 | CGTCCCAGACTACGCGGCCGCTTCGGACACGTGGAGCTCTATCC | CTGATCAGCGGGTTTAAACCTATAAATTCTTAGGTTTAGAGATGATACCATCTGGG |
| pEFtak-WTAPΔNLS-FLAG | CTTGGTACCATGAGTGAAACAGACTTCAAAGTTATGGCAAG | TTATAATCAGCGGCCGCCAAAACTGAACCCTGTACATTTACAC |
| pEFtak-WTAPΔMETTL3-FLAG | AGGTAAGCTTGGTACCATGTTCCTAAAAATGAAAGGTGAACTGGAACAG | AGGTAAGCTTGGTACCATGTTCCTAAAAATGAAAGGTGAACTGGAACAG |
| pLEX-WTAPΔMETTL3-HA | CCGACTCTACTAGAGGATCCGCCACCATGTTCCTAAAAATGAAAGGTGAACTGGAAC | CCGACTCTACTAGAGGATCCGCCACTGTTCCTAAAAATGAAAGGTGAACTGGAAC |