FIG 3.

The H240R protein (pH240R) inhibits TNF-α- or LPS-induced proinflammatory cytokine expression. (A and B) pH240R inhibited TNF-α- or LPS-induced proinflammatory cytokine expression. HEK293T cells were transfected with pCAGGS-Flag or pFlag-H240R for 24 h, followed by treatment with or without TNF-α (100 μg/mL) (A) or LPS (100 μM) (B) for another 10 h, and the transcription levels of IL-1β, IL-6, and TNF-α were measured by RT-qPCR. (C and D) pH240R inhibited TNF-α- or LPS-induced proinflammatory cytokine expression. PAMs were transfected with pCAGGS-Flag or pFlag-H240R for 24 h, followed by treatment with or without TNF-α (100 μg/mL) (A) or LPS (100 μM) (B) for another 10 h, and the transcription levels of IL-1β, IL-6, and TNF-α were measured by RT-qPCR. Data are from three independent experiments. The error bars denote standard errors of the means. The significance of the difference between groups (n = 3) was determined using Student’s t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).