TABLE 1.
Primers used in this studya
| Primer | Sequence (5′–3′) | Description |
|---|---|---|
| Flag-NLRP3-F | ATTTTGGCAAAGAATTCATGAGCATGGCAAGCG | For NLRP3 |
| Flag-NLRP3-R | CACGATGCGGCCGCTCGAGTTACTTAGACGTTT | |
| Flag-ASC-F | GAATTCGCCACCATGGGGTGCACGCGTGACG | For ASC |
| Flag-ASC-R | TCACAGATCCTCTTCAGAGATGAGTTTCTGCTC | |
| Flag-caspase 1-F | GAATTCATGGCCGATAAGGTGCTGAAGGAGGA | For caspase 1 |
| Flag-caspase 1-R | CTCGAGTTAATGTCCTGGGAAGAGATAAAAAGA | |
| Flag-pro-IL-1β-Gluc-F | GGTACCGCCACCATGGCCATAGTACCTGAA | For pro-IL-1β/Gluc |
| Flag-pro-IL-1β-Gluc-R | CTCGAGTCACTTATCGTCGTCACCACCGGCCC | |
| EGFP-IκBα-F | GGCAAAGAATTCATGTTCCAGCCCGCAGAGCC | For IκBα |
| EGFP-IκBα-R | CACGATGCGGCCTCATAACGTCAGGCGCT | |
| Myc-IKKα-F | CTTATGGCCATGGAGGCCCGAATTCGG | For IKKα |
| Myc-IKKα-R | GCGGTACCTCGAGTCATCCTGTTAACCAACT | |
| Myc-IKKβ-F | TGGAGGCCCGAATTCGGATGAGCTGGTCACCT | For IKKβ |
| Myc-IKKβ-R | GCGGTACCTCGAGTCACGAGGCCTGCTCCAGG | |
| Myc-NEMO-F | ATGAGCAGGACCCCCTGGAAGAATTCGG | For NEMO |
| Myc-NEMO-R | GCGGTACCTCGAGCTACTCGATACACTCCAT | |
| NLRP3-D1-Myc-F | ATGGAGGCCCGAATTCGGATGAGCATGGCAAG | For NLRP3-D1 |
| NLRP3-D1-Myc-R | CGGCCGCGGTACCTCGAGTTACACAGGCTCAG | |
| NLRP3-D2-Myc-F | AGCCTGTGTAACTCGAGGTACCGCGGCCGCGG | For NLRP3-D2 |
| NLRP3-D2-Myc-R | GCGGCCGCGGTACCTCGAGTTACCAGTTATTG | |
| NLRP3-D3-Myc-F | ATGGAGGCCCGAATTCGGATGTGGGACAATGC | For NLRP3-D3 |
| NLRP3-D3-Myc-R | GCGGCCGCGGTACCTCGAGTTACTGGGAAGGC | |
| NLRP3-D4-Myc-F | ATGGAGGCCCGAATTCGGATGGAAAAGGAGGA | For NLRP3-D4 |
| NLRP3-D4-Myc-R | CGGCCGCGGTACCTCGAGTTACTGGGAAGGC | |
| IKKα-F | CAATGGAATATTGTTAC | RT-qPCR for IKKα |
| IKKα-R | GGATTCGATATTTGCAT | |
| IKKβ-F | GCCAGGGCGGGGATCTGC | RT-qPCR for IKKβ |
| IKKβ-R | TCTGTTTTCGTGAAGGTA | |
| NEMO-F | GTTCTGCGAGGCCAGGCG | RT-qPCR for NEMO |
| NEMO-R | TGCTCCTTGTCCTCTGCCA | |
| IκBα-F | TGCACTTGGCCATCATCCA | RT-qPCR for IκBα |
| IκBα-R | GCTCAGGATCACAGCCA | |
| NLRP3-F | GTACCTTTCCAGAATCTCTA | RT-qPCR for NLRP3 |
| NLRP3-R | GTTCACACTCTCACCCA | |
| TNF-α-F | CCTACTGCACTTCGAGGTTATC | RT-qPCR for TNF-α |
| TNF-α-R | ACGGGCTTATCTGAGGTTTG | |
| IL-1β-F | ACCCAAAACCTGGACCTTGG | RT-qPCR for IL-1β |
| IL-1β-R | CATCACAGAAGGCCTGGGAG | |
| IL-6-F | CTCATTAAGTACATCCTCGG | RT-qPCR for IL-6 |
| IL-6-R | GTCTCCTGATTGAACCCAGA | |
| GAPDH-F | GAAGGTCGGAGTGAACGGATTT | RT-qPCR for GAPDH |
| GAPDH-R | TGGGTGGAATCATACTGGAACA |
a
F, forward; R, reverse.