FIG 2.

(A to D) The negative regulation of eIF4A3 on IFN-β, NF-κB, IRF3, and ISRE promoter. HEK293T cells were transfected with increasing amounts of Flag-eIF4A3 or sieIF4A3 and the IFN-β/NF-κB/IRF3/ISRE luciferase reporter. Then, 24 h after transfection, the cells were stimulated by SeV for 10 h and measured by the luciferase assays. eIF4A3 knockdown A549 cells or WT A549 cells were transfected with the IFN-β/NF-κB/IRF3/ISRE luciferase reporter. The cells were stimulated with SeV for another 10 h after 24 h posttransfection and measured by the luciferase assays. For all experiments described above, the data are presented as the mean ± SD of three independent experiments (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; two-tailed Student’s t test).