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. 2022 Oct 31;96(22):e01513-22. doi: 10.1128/jvi.01513-22

FIG 3.

FIG 3

The inhibition of eIF4A3 on the transcription of IFN-β and ISGs. (A and B) Overexpression of eIF4A3 inhibited the mRNA of IFN-β, ISGs, and cytokines. A549 cells were transfected with HA-eIF4A3 or control vector (HA), followed by stimulation with or no stimulation with poly(I·C) (100 ng) for 6 h. The mRNA levels of the indicated target genes were measured by qRT-PCR. (C and D) sieIF4A3 promoted the mRNA of IFN-β, ISGs, and cytokines. A549 cells were treated with negative-control RNAs (NC) and siRNAs targeting eIF4A3 (sieIF4A3) for 24 h, and then cells were both induced or uninduced by poly(I·C) (100 ng) for 6 h. The mRNA levels of the indicated target genes were detected by qRT-PCR. All the mRNA levels were normalized to the GAPDH level (mean ± SD of three independent experiments; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; two-tailed Student’s t test).