TABLE 1.
Primers used for PCR
| Primer name | Sequences (5′ to 3′)a |
|---|---|
| Flag-eIF4A3 | F: CGGAATTCAATGGCTACAAACTTCCTAG |
| R: GCTCTAGAGATCTTGTTGAAAGCTGC | |
| Myc-eIF4A3 | F: GCGTCGACCATGGCGACCACGGCCACGAT |
| R: AAGAATGCGGCCGCATAAGATCAGCAACGTTCATCGGCATC | |
| HA-eIF4A3 | F: CCATCGATATGGCGACCACGGCCAC |
| R: CCCTCGAGTTAGATAAGATCAGCAACGTTCATCG | |
| HA-eIF4A3-Nt | F: CCATCGATATGGCGACCACGGCCACGA |
| R: CCCTCGAGTTCATCACGTTTCACCAAGATGCGGA | |
| HA-eIF4A3-Ct | F: CCATCGATTTGACTCTGGAAGGCATCAAGCAAT |
| R: CCCTCGAGGATAAGATCAGCAACGTTCATCGGC | |
| HA-eIF4A3(DEAD-DQAD) | F: TGGATCAAGCTGATGAAATGTT |
| R: AGCTTGATCCAAAACCAACATT | |
| HA-eIF4A3(Δ motif I) | F: CCATCGATATGGCGACCACGGCCAC |
| R: ACTGAGATACTGAAGGTGGCGATGACATCTCTCCCTTTGA | |
| F: TCAAAGGGAGAGATGTCATCGCCACCTTCAGTATCTCAGT | |
| R: CCCTCGAGGATAAGATCAGCAACGTTCATCG | |
| HA-RIG-I-N | F: GGGGTACCATGACCACCGAGCAGCGAC |
| R: CCCTCGAGTTTAAATGGGCTGTACAAGTTTGTATC | |
| HA-IRF3 | F: CGGAATTCATGGGAACCCCAAAGCCACGGATC |
| R: CCCTCGAGGCTCTCCCCAGGGCCCTGGAAAT | |
| HA-TBK1 | F: CCCATCGATATGCAGAGCACTTCTAATCATCTGT |
| R: CCGCTCGAGCTAAAGACAGTCAACGTTGCGAA | |
| Flag-EGFP | F: GGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCCATGGTGAGCAAGGGCGAGGAGCT |
| R: GCTCTAGACTTGTACAGCTCGTCCATGCCGAGAGT | |
| Flag-EGFP-ΔIRF3 | F: CCGGAATTCAATGCGGGTAGGGGGTGCCTCCTCCCT |
| R: ACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCTCTCCCCAGGGCCCTGGAAAT |
a
The gene sequences are downloaded from NCBI. F, forward; R, reverse.