TABLE 2.
Distribution of viral particles
| BAC | No. of particlesa | Value (%) for: |
||||
|---|---|---|---|---|---|---|
| Nucleusb | Perinuclear spacec | Cytoplasm |
Cell surfacef | |||
| Unenveloped capsidsd |
Enveloped virionse |
|||||
| WT | 1294 | 549 (42.43) | 12 (0.93) | 442 (34.16) | 215 (16.61) | 76 (5.87) |
| ORF33 DN17 | 1255 | 643 (51.24) | 23 (1.83) | 589 (46.93) | 0 (0)****g | 0 (0)****g |
| ORF33 DN17.R | 1227 | 663 (54.03) | 20 (1.63) | 306 (24.94) | 211 (17.20) | 27 (2.20)*g |
a
Total viral and subviral particles enumerated in 14 selected cells that contained viral particles.
b
Values are calculated as number of nucleocapsids/total number of viral particles.
c
Values are calculated as number of perinucleus capsids/total number of viral particles.
d
Values are calculated as number of immature virions (including capsids) in the cytoplasm/total number of viral particles.
e
Values are calculated as number of mature virions in the cytoplasm/total number of viral particles.
fValues are calculated as number of fully mature extracellular virions/total number of viral particles.
g
*, P < 0.05; ****, P < 0.0001 versus WT, unpaired t test.