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. 2022 Nov 20;14(1):2143222. doi: 10.1080/19490976.2022.2143222

Figure 2.

Figure 2.

BmΔvjbR is engineered to produce indole. a, Schematic representation of the engineered BmΔvjbR::tnaA harboring a plasmid carrying a tnaA expression cassette. The indole biosynthesis pathway is depicted in the figure. TnaA catalyzes the conversion of tryptophan to indole. b, Mass spectrometric analysis of indole production by engineered BmΔvjbR::tnaA. c, Western blotting analysis of the expression of tnaA protein in the parental strain compared with the engineered BmΔvjbR::tnaA strain. Graphical representation of the comparative analysis of indole production by BmΔvjBR parental bacterial strain and the engineered BmΔvjbR::tnaA strain. d, Colonization of engineered BmΔvjbR::tnaA in the spleen, liver, kidney and lymph-nodes of CIA mice. The bacteria colonized in all the organs for 3 days post-inoculation and could be observed only in the spleen for 7 days. The numbers represent single colonies grown on the plate. The bacteria recovered at 7 days post infection from mice still could produce indole. e, Serum ELISA analysis of anti-Brucella IgG production. The positive and negative controls were used as per the manufacturer’s instructions. f, Indole production of BmΔvjbR::tnaA bacteria recovered from CIA mice. Data represents means ± SD. Student’s t-test or Tukey’s multiple comparisons test was applied for statistical analysis. *, ***: significance at p < 0.05, 0.001.