Skip to main content
. 2022 Jul 14;16(11):1751–1761. doi: 10.1093/ecco-jcc/jjac084

Figure 5.

Figure 5.

AC-73 inhibits activation of the CD147/ERK1/2 and STAT3 signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], *p<0.05, **p<0.01 from a Mann–Whitney test.