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. Author manuscript; available in PMC: 2022 Nov 23.
Published in final edited form as: Nat Microbiol. 2021 Sep 23;6(10):1319–1333. doi: 10.1038/s41564-021-00964-2

Extended Data Fig. 6 |. TBC1D5 promotes lysosomal targeting of M2 protein.

Extended Data Fig. 6 |

(a) 293 T cells were treated with negative control scrambled siRNA or siTBC1D5 for 48 h. Cells were then infected with A/WSN/33 (MOI 2), and at 8 and 16 h p.i. cells were lysed and levels of TBC1D5, NS1, and β-actin were analysed using SDS–PAGE. Blot is representative of two independent experiments. (b) 293 T cells were transfected with indicated siRNAs followed by infection with A/WSN/33 (MOI 1). At 8, 16 and 24 h p.i., cells were subjected to immunolabeling with anti-M2 in the absence of permeabilization agent (surface M2), and M2 relative fluorescence mean intensity levels were recorded by flow cytometry. Data represent mean ± s.d. of two independent experiments (n = 2). Statistical significance was calculated using two-way ANOVA with Bonferroni’s multiple comparisons post hoc test.