FIG. 5.
Total IgG antibodies (A) specific for L. major (white bars) or T. gondii (black bars) antigens and (B) specific IgG1 (white bars) or IgG2a (black bars) isotypes against L. major in mice infected or not with T. gondii and challenged with L. major. Mice were infected with 20 cysts of T. gondii and 5 days later were challenged with 106 stationary forms of L. major in the hind footpads. Mice were sacrificed 7 weeks later, and sera were obtained for total IgG and IgG isotype assays by enzyme-linked immunosorbent assay. Bars represents means (± standard deviations) of eight mice per group. These data are pooled from two separate experiments.