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. 2022 Nov 9;12:938286. doi: 10.3389/fcimb.2022.938286

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Copyright © 2022 Tan, Tan, Zhang, Li, Ji, Fang, Wu, Rao, Zeng and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction and confirmation of the deletion mutant strain ΔapuA. (A) Genetic map of the loci encoding ApuA in the S. suis genome. Two gray bars represent the coding regions of α-amylase and pullulanase of apuA, respectively. Numbers 1–4 represent the detection primer pairs apuR-F/apuR-R, amy-F/amy-R, pul-F/pul-R, and sgaT-F/sgaT-R, respectively. (B) Combined PCR analyses of ΔapuA. Numbers 1–4 represent the specific DNA fragments amplified with corresponding primers in Figure 2A . M represents the DNA marker (100–2000 bp). (C) Confirmation of the transcriptional inactivation of apuA by real-time PCR. Numbers 1-4 represent the specific DNA fragments amplified with corresponding primers in Figure 2A . M represents the DNA marker.