Table 1.
Bacterial strains and plasmids used in this study.
| Strains/plasmids | Characteristics a | Source |
|---|---|---|
| Bacterial strains | ||
| SC19 | Virulent Chinese S. suis serotype 2 isolate; wild-type (WT) | (Tan et al., 2015) |
| ΔapuA | SC19 apuA::erm (Ermr) | This study |
| E. coli DH5α | Cloning host for recombinant plasmids | Trans |
| Plasmids | ||
| pAT18 | The plasmid carrying an erythromycin resistance rRNA methylase (erm) gene expression cassette | (Trieu-Cuot et al., 1991) |
| pSET4s | Temperature-sensitive E. coli- S. suis shuttle vector (Spcr) | (Takamatsu et al., 2001) |
| pSET4s-apuA | Derived from pSET4s for knocking out apuA in SC19 (SpcrErmr) | This study |
a Ermr, erythromycin resistant; Spcr, spectinomycin resistant.