Table 2.
Validation of RNA-Seq results by real-time quantitative PCR (qRT-PCR).
| Gene locus tag | Gene | Description | Fold change of RNA-Seq | Fold change of qRT-PCR |
|---|---|---|---|---|
| SSUSC84_0015 | ftsH | ATP-dependent zinc metalloprotease | -3.45 | -4.76 ± 1.03 |
| SSUSC84_0092 | rpmJ | 50S ribosomal protein L36 | -7.14 | -12.50 ± 1.40 |
| SSUSC84_0249 | adhP | Alcohol dehydrogenase | 76.28 | 61.15 ± 3.51 |
| SSUSC84_0270 | dnaJ | Molecular chaperone | 7.58 | 2.84 ± 0.65 |
| SSUSC84_0376 | cysK | Cysteine synthase A | -6.25 | -5.88 ± 1.12 |
| SSUSC84_0671 | mrp | MucBP domain-containing protein | -9.09 | -25.06 ± 1.01 |
| SSUSC84_0917 | glgD | Glucose-1-phosphate adenylyltransferase subunit | 25.54 | 23.47 ± 2.86 |
| SSUSC84_1079 | citZ | Citrate synthase | -16.67 | -33.33 ± 1.82 |
| SSUSC84_1262 | – | PTS transporter subunit EIIC | 16.99 | 17.15 ± 1.25 |
| SSUSC84_1724 | msmK | ABC transporter ATP-binding protein | 5.81 | 2.98 ± 0.59 |
| SSUSC84_1871 | apuA | Surface-anchored amylopullulanase | 65.44 | 43.57 ± 2.07 |
| SSUSC84_1959 | mnmG | tRNA uridine-5-carboxymethylaminomethyl synthesis enzyme | -5.26 | -6.25 ± 1.04 |