Figure 5.

Distribution of ASD gene expression among cell types of the prenatal human cerebellum. To identify the cell type specificity of ASD gene expression, we compared the expression of each gene in a cell type relative to its expression in all other cell types using Seurat v4 [118] and the prenatal human cerebellum dataset [109]. This analysis revealed that 238 of the 345 ASD genes were significantly expressed in distinct cell types (average logFC >1.5 and FDR < 0.05). Overlapping expression of ASD genes among the top 9 cell types of the prenatal cerebellum is indicated by the numbers of genes (x-axis) and the numbers of genes per cell type (y-axis; circles with connecting lines indicate genes expressed in additional cell types); cell types with <5 genes expressed were omitted from the display. Colors correspond to cell type expression for the genes included in the protein-protein network, as shown in Figure 6.