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. 2022 Nov 23;8(47):eadd6391. doi: 10.1126/sciadv.add6391

Fig. 2. Expression of NOTCH2NLC-(GGC)98 produces multiple polypeptides in NOTCH2NLC transgenic mice in an age-dependent manner.

Fig. 2.

(A) Immunofluorescent staining against Flag (polyG), HA (polyA), and Myc (polyR) in the brain of NOTCH2NLC-(GGC)98 mice at P50. The intranuclear or perinuclear polyG, polyA, and polyR inclusions were observed under higher magnification in the hippocampus of NOTCH2NLC-(GGC)98 mouse. Green, Flag/HA/Myc; blue, DAPI. Scale bar, 50 μm. (B) Electron microscopy of cerebellar neurons from the NOTCH2NLC-(GGC)17 and NOTCH2NLC-(GGC)98 mice at P50. Yellow arrow indicates the intranuclear inclusions. Scale bar, 2 μm. (C and D) Western blotting against Flag (polyG), HA (polyA), and Myc (polyR) tag (C) and PEP122 and PEP123 (D) in the cortex from control and NOTCH2NLC-(GGC)98 mice at P50. (E) Immunofluorescent staining against Flag (polyG) and HA (polyA) in cortex of NOTCH2NLC-(GGC)98 mice at P50. Green, Flag; magenta, HA. Scale bar, 10 μm. (F) Immunofluorescent staining against Flag (polyG) in the different brain regions of NOTCH2NLC-(GGC)98 mice at P12 and P32. Green, Flag; blue, DAPI. Magenta arrows indicate the intranuclear inclusions in the cerebellum. Scale bar, 50 μm. (G) Western blotting against Flag (polyG) and HA (polyA) in the cortex from NOTCH2NLC-(GGC)17 and NOTCH2NLC-(GGC)98 mice at P12 and P32.