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. 2022 Nov 23;8(47):eade1942. doi: 10.1126/sciadv.ade1942

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Fig. 4. — (A) Genomic structure of the mouse EPAS1 region (chr17: 86,797,164 to 86,807,311, mm10) in a segment spanning the orthologous ENH5 enhancer. Red bars denote the location of CRISPR guide RNAs that flank mENH5. (B) ENH5 is conserved across multiple mammalian species as well as opossum and chicken at the sequence level. (C) ENH5 is functionally conserved between human and mouse. Luciferase results for mENH5 activity in kidney (HEK293), cardiomyocytes (HL-1), and endothelial (teloHAEC) cells are shown. The plots show means + SEM from three independent construct DNA preparations, each with three independent transfection replicates. * ***P < 0.005.