Table 1. Formulations of LNP, CNE, and Lipo.
DMG-PEG2000, 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3-N,N,N-trimethylammonium; DSPC, 1,2-dioctadecanoyl-sn-glycero-3-phophocholine; DSPE-PEG2000, distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000].
| Nanocarrier | Lipids | Composition (mass ratio) | Lipid concentration (mg/ml) | N/P ratio with mRNA |
| LNP | DLin-MC3-DMA:DMG-PEG2000:cholesterol:DSPC | 13:1.6:6.2:3.3 | 1.205 | 6 |
| CNE | DOTAP:lipoid E-80:vitamin E | 6:7:7 | 33.33 | 4.5 |
| Lipo | DOTAP:DOPE:DSPE-PEG2000:DSPE-PEG2000-Pardaxin | 1552:388:15:45 | 4 | 3.4 |