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. 2022 Nov 23;8(47):eabq1984. doi: 10.1126/sciadv.abq1984

Fig. 6. ACC is required for LPS-induced rewiring of the macrophage lipidome.

Fig. 6.

(A) Schematic of lipidomic analysis workflow from flox or ACCΔLysM BMDMs stimulated with LPS (100 ng/ml) for 6 hours. (B) Volcano plots representing fold change of lipid species abundance in flox or ACCΔLysM BMDMs stimulated with LPS (100 ng/ml) for 6 hours (n = 6). (C) Heatmap of significantly increased phosphatidylethanolamine (PE) species in LPS-stimulated flox and ACCΔLysM BMDMs, relative to respective unstimulated cells (n = 6). (D) Heatmap of significantly increased phosphatidylcholine (PC) species in LPS-stimulated flox and ACCΔLysM BMDMs relative to respective unstimulated cells (n = 6). (E) Levels of 16:0/20:4 phosphatidylcholine in flox or ACCΔLysM BMDMs stimulated with LPS (100 ng/ml) for 6 hours (n = 6). (F) Schematic showing fates of acetyl-CoA in the de novo lipogenesis and sterol biosynthesis pathways. (G) Levels of cholesterol in flox or ACCΔLysM BMDMs stimulated with LPS (100 ng/ml) for 6 hours (n = 4 to 6). Data are represented as means ± SEM (E and G). Significance determined by two-tailed Student’s t test (C and D) or one-way ANOVA (E and G). *P < 0.05, **P < 0.01, and ***P < 0.001. (A) Created using BioRender.com. See also fig. S6, related to Fig. 6.