Table 2. Selected thermodynamic parameters of the six Kyrpides, Ouzounis, Woese (KOW) domains.
The values were derived from thermal denaturations monitored by differential scanning calorimetry (DSC) and circular dichroism (CD) spectroscopy. Standard deviations result from data fitting.
| Parameter | EcNusG-KOW | MtNusG-KOW | MjSpt5-KOW | hSpt5-KOW5 | EcRfaH-KOW | VcRfaH-KOW |
|---|---|---|---|---|---|---|
| Tm (°C) pH 7/pH 4 | ||||||
| CD | –*/– | 76.6±0.874/– | –†/–† | –/60.5±0.771 | 50.3±0.388/– | 65.2±1.78/– |
| DSC | 87.0±0.0485/– | 77.0±0.0885/– | 111±0.0326/– | –/58.0±0.162 | 47.3±0.143/– | 70.2±0.379/– |
| ΔHu (Tm) (kJ/mol) pH 7/pH 4 | ||||||
| CD | –*/– | 193±11.3/– | –†–/–† | –/140±12.4 | 121±5.15/– | 162±2.91/– |
| DSC | 222±0.339/– | 192±0.417/– | 293±0.345/– | –/117±0.735 | 129±0.432/– | 169±1.56/– |
| ΔCp (Tm) (kJ/(K mol)) pH 7/pH 4 |
0.800/– | 0.346/– | –*/– | –/2.27 | 2.18/– | 0.148/– |
*
Data was not fitted due to the lack of the baseline of the unfolded state.
†
No denaturation.