Table 5. Exchange parameters derived from global fitting of the chemical exchange saturation transfer (CEST) experiments to a two-state exchange model.
| Parameter | hSpt5-KOW5 | VcRfaH-KOW | EcRfaH-KOW |
|---|---|---|---|
| pA (%) | 99.15±0.02 | 99.57±0.01 | 94.47±0.46 |
| pB (%) | 0.85±0.02 | 0.43±0.01 | 5.53±0.46 |
| kAB (s–1) | 0.76±0.03 | 0.32±0.02 | 0.82±0.10 |
| kBA (s–1) | 88.62±3.12 | 74.24±3.17 | 13.98±1.24 |
| kex (s–1) | 89.38±3.15 | 74.57±3.18 | 14.80±1.31 |
| τA (s) | 1.31±0.05 | 3.08±0.15 | 1.22±0.15 |
| τB (ms) | 11.28±0.40 | 13.47±0.57 | 71.52±6.33 |
| ΔG (kJ/mol) | 11.81±0.05 | 13.48±0.07 | 7.18±0.21 |