Table 12.

Affinity and stability matured antibodies designed by Rosetta based on Bamlanivimab scaffold.

Chain	Target positions <0.7 (nm)	Model	Chain	Designed positions	Score (REU)
H	28, 29, 30, 31, 32, 33, 47, 50, 52, 54, 55, 57, 58, 59, 60, 61, 65, 74, 100, 101, 102, 103, 104, 105, 106, 109, 110	1	H	S30T, R50M, I54V, N59A, A103Y, Y110F	−1315.52
			L	Q27S, S28P, S30D, S91T, T94M, R96W
		2	H	S30T, R50M, I54V, N59A, K74Q, A103Y, Y110F	−1314.53
			L	Q27S, S28P, S30D, S91T, T94M, R96W
		3	H	S30T, R50M, I54V, N59A, A103Y, Y106W, Y110F	−1312.68
			L	Q27S, S28P, S30D, Y32F, S91T, T94M, R96W
		4	H	S30T, R50M, I54V, N59A, A103Y, Y110H	−1309.7
L	2, 27, 28, 29, 30, 32, 91, 92, 93, 94, 98		L	Q27S, S28P, S30D, S91T, T94M, R96W
		5	H	S30T, R50M, I54V, A58V, N59A, A103Y, Y110F	−1307.98
			L	Q27S, S28P, S30D, S91T, T94M, R96W
		6	H	S30T, N31D, Y32A, R50M, I54V, N59A, A103Y, R104K, H105N, Y110F	−1305.94
			L	Q27S, S28P, S30D, S91T, T94M, R96W
		7	H	S30T, R50M, I54V, N59A, A103Y, Y110F	−1305.49
			L	Q27T, S28E, S30G, S91T, T94M, R96W