Table 3. Comparison of mRNA content in leukocytes for the control and patient with acute appendicitis.
| Control Median (min–max) |
Patients with AA Median (min–max) |
Fold | p* | |||
|---|---|---|---|---|---|---|
| ROCK1 | ||||||
| Total (n=93) | ΔCT=Ct(target)–Ct(housekeeping) | 1.22 (0.02–7.01) |
2.11 (0.01–42.08) |
ΔΔCT: 1.22–2.11= -0.89 | 0.0027 | |
| Content=2−ΔCT | 0.43 (0.01–0.98) |
0.23 (0.00–0.99) |
Fold: 0.43/0.23=1.87 | |||
| Male (n=67) | ΔCT=Ct(target)–Ct(housekeeping) | 1.19 (0.02–7.01) |
2.01 (0.01–42.08) |
ΔΔCT: 1.19–2.01=-0.82 | 0.0008 | |
| Content=2−ΔCT | 0.44 (0.01–0.98) |
0.25 (0.00–0.99) |
Fold: 0.44/0.25=1.76 | |||
| Female (n=26) |
ΔCT=Ct(target)–Ct(housekeeping) | 1.79 (0.02–4.66) |
2.18 (0.01–6.75) |
ΔΔCT: 1.79–2.18= -0.39 | 0.5252 | |
| Content=2−ΔCT | 0.29 (0.04–0.98) |
0.22 (0.01–0.99) |
Fold: 0.29/0.22=1.32 | |||
| ROCK2 | ||||||
| Total (n=93) | ΔCT=Ct(target)–Ct(housekeeping) | 1.40 (0.23–21.93) |
1.20 (0.15–40.09) |
ΔΔCT:1.40–1.20=0.20 | 0.0570 | |
| Content=2−ΔCT | 0.38 (0.00–0.85) |
0.43 (0.00–0.90) |
Fold: 0.38/0.43=0.88 | |||
| Male (n=67) | ΔCT=Ct(target)–Ct(housekeeping) | 1.49 (0.24–21.93) |
1.12 (0.15–40.09) |
ΔΔCT:1.49–1.12=0.37 | 0.0719 | |
| Content=2−ΔCT | 0.36 (0.00–0.85) |
0.46 (0.00–0.90) |
Fold: 0.36/0.46=0.78 | |||
| Female (n=26) | ΔCT=Ct(target)–Ct(housekeeping) | 1.20 (0.23–4.61) |
1.29 (0.15–3.85) |
ΔΔCT:1.20–1.29= -0.09 | 0.4671 | |
| Content=2−ΔCT | 0.43 (0.04–0.85) |
0.41 (0.07–0.90) |
Fold: 0.43/0.41=1.05 | |||
ROCK: Rho-kinase. The results are presented as median (IQR).
*
Mann-Whitney U test.