Table 2.
Total phenolics and total flavonoid contents of immature and mature leaf extracts of B. balsamifera using different eluting solvents.
| Treatment | TPC (mg GAE/g DW) | TFC (mg QE/g DW) | |
|---|---|---|---|
| Maturity | Solvent | ||
| Immature | 44.47 ± 41.16a | 110.30 ± 96.55a | |
| Mature | 30.94 ± 34.93b | 86.94 ± 89.43b | |
| F-test (maturity) | ∗∗ | ∗∗ | |
| Water | 25.74 ± 8.95b | 67.07 ± 18.95b | |
| 50% EtOH | 98.14 ± 12.84a | 250.31 ± 25.59a | |
| 95% EtOH | 25.24 ± 9.29b | 45.56 ± 2.48c | |
| Hexane | 1.68 ± 0.53c | 31.53 ± 3.67d | |
| F-test (solvent) | ∗∗ | ∗∗ | |
| F-test (maturity ∗ solvent) | ∗∗ | ns | |
| Immature | Water | 33.66 ± 3.14c | 84.33 ± 1.80c |
| 50% EtOH | 108.69 ± 7.48a | 267.18 ± 8.14a | |
| 95% EtOH | 33.46 ± 2.83c | 56.09 ± 4.57d | |
| Hexane | 2.06 ± 0.33e | 33.61 ± 3.84ef | |
| Mature | Water | 17.83 ± 1.66d | 49.81 ± 0.97de |
| 50% EtOH | 87.59 ± 4.74b | 233.44 ± 26.77b | |
| 95% EtOH | 17.02 ± 2.25d | 35.04 ± 2.48ef | |
| Hexane | 1.30 ± 0.40e | 29.45 ± 2.42f | |
| F-test | ∗∗ | ∗∗ | |
| C.V. (%) | 9.58 | 10.36 | |
The data are expressed as mean ± SD (n = 3). Values in the same column followed by different letters indicate significant differences at P < 0.05 according to Duncan's multiple range test; ns = not significant; ∗∗ = significant at P < 0.01 level.