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. Author manuscript; available in PMC: 2022 Nov 24.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1088–1100.e5. doi: 10.1016/j.celrep.2019.12.094

Figure 4. IPCs fail to facilitate epithelial repair upon intestinal challenge with V. cholerae.

Figure 4.

(A) Genes that regulate IPC growth and cell cycle from RNA-seq of IPCs of flies mock infected or infected with C6706 or C6706ΔvasK. (B) Immunofluorescence of the posterior midguts of esgts>GFP flies mock infected or infected with Ecc15, C6706ΔvasK, C6706ΔvipA, or C6706. Hoechst marks DNA (blue) and GFP marks esg positive IPCs (green). Scale bars are 10μm. (C) Quantification of the number of IPCs per unit surface area from (B). Each dot represents a measurement from a single fly gut. (D-E) Quantification of the number of PH3 positive cells in the posterior midguts of (D) w1118 or (E) OregR flies that were mock infected or infected with C6706ΔvasK, or C6706.