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. Author manuscript; available in PMC: 2022 Nov 24.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1088–1100.e5. doi: 10.1016/j.celrep.2019.12.094

Figure 5. Impaired IPC differentiation in response to the T6SS.

Figure 5.

(A) Differentially regulated genes in the Notch signaling pathway, from RNA-sequencing of IPCs from flies mock infected or infected with C6706ΔvasK or C6706 (B) Immunofluorescence of the posterior midguts of esgts>CFP, Su(H)-GFP flies mock infected or infected with C6706ΔvasK, or C6706. DRAQ5 marks DNA (red), CFP marks esg positive IPCs (blue), and GFP marks Su(H) positive enteroblasts. Scale bars are 10μm. (C) Quantification of the number of IPCs (CFP-positive), per unit surface area from (B). Each dot represents a measurement from a single fly gut. (D) Quantification of the number of intestinal stem cells (CFP-positive, GFP-negative) per unit surface area from (B). (E) Quantification of the number of enteroblasts (CFP-positive, GFP-positive) per unit surface area from (B).