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. Author manuscript; available in PMC: 2022 Nov 24.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1088–1100.e5. doi: 10.1016/j.celrep.2019.12.094

Figure 6. IPC suppression of growth in response to the T6SS requires intestinal symbionts.

Figure 6.

(A) Immunofluorescence of the posterior midguts of germ free esgts>GFP flies mock infected or infected with C6706ΔvasK, or C6706. Hoechst marks DNA (blue) and GFP marks esg positive IPCs (green). Scale bars are 10μm. (B) Quantification of the number of IPCs per unit surface area from (A). Each dot represents a measurement from a single fly gut. (C) Quantification of the number of IPCs per unit surface area in esgts >GFP flies infected with C6706. Flies were made germ-free either by the administration of antibiotics to adults (antibiotic) or by bleaching of embryos (axenic).