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. Author manuscript; available in PMC: 2022 Nov 24.
Published in final edited form as: Cell Rep. 2020 Jan 28;30(4):1088–1100.e5. doi: 10.1016/j.celrep.2019.12.094

Figure 7. T6SS suppression of epithelial renewal requires higher-order microbiome interactions.

Figure 7.

Immunofluorescence of posterior midguts of (A,D,G) CR, (B) Lb mono-associated, (E) Ab mono-associated, or (H) poly-associated esgts >GFP flies mock infected or infected with C6706ΔvasK, or C6706. Hoechst marks DNA (blue) and GFP marks esg positive IPCs (green). Scale bars are 10μm. Quantification of the number of IPCs per unit surface area in the guts of (C,F,I) CR, (C) Lb mono-associated, (F) Ap mono-associated, or (I) poly-associated flies. 2–3 day old virgin female flies were raised on antibiotics 5 day at 25°C to eliminate the microbiome. Germ free flies were then associated with microbial populations as indicated.