Extended Data Fig. 6. Transcriptional control of cold-stimulated futile creatine cycling gene expression.

a-b, RT-qPCR from BAT of male (a) Esrra/g^AdipoqCre and Esrra/g^fl/fl mice (6 °C for 24 hours) (n = 5 per group) and (b) Ebf1/2^AdipoqCre and Ebf1/2^fl/fl mice (4 °C for 7 days) (n = 3 for Ebf1/2^AdipoqCre at 4 °C; n = 4 for all other groups). c-d, Western blot analysis of BAT from (c) male (Esrra/g^fl/fl: n = 2 for 30 °C, n = 3 for 6 °C) (Esrra/g^AdipoqCre: n = 3 for 30 °C, n = 4 for 6 °C) and (d) female mice (n = 3 per group) subjected to 30 °C or 6 °C for 24 hours. e-f, Western blot quantification from (e) Extended Data Fig. 6c (Esrra/g^fl/fl: n = 2 for 30 °C, n = 3 for 6 °C) (Esrra/g^AdipoqCre: n = 3 for 30 °C, n = 4 for 6 °C) and (f) Extended Data Fig. 6d (n = 3 per group). g, RT-qPCR from BAT of Ppargc1a^Ucp1CreERT2 and Ppargc1a^fl/fl mice, reared at 22 °C, housed at 30 °C for 5 days and then subjected to 30 °C or 6 °C for 24 hours (n = 4 per group). h, Western blot quantification from Fig. 1r (n = 3 per group). i, Western blot of BAT from male mice (6–8 weeks of age), treated as in Extended Data Fig. 6g. j, Western blot quantification from Extended Data Fig. 6i (n = 3 per group). k, Western blot analysis of BAT from female mice (6–8 weeks of age), reared at 22 °C, housed at 30 °C for 5 days and then injected i.p. (5 injections over 48 hours) with CL 316,243 (1 mg kg⁻¹) or saline at 30 °C (n = 3 per group). l, Western blot quantification from Extended Data Fig. 6k (n = 3 per group). Data are presented as mean ± s.e.m. and n numbers are of biologically independent experiments. a, b, g, multiple two-tailed student’s t-tests (Holm-Šidák test); e, f, h, j, l, Two-way ANOVA (Fisher’s LSD).

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