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. 2022 Nov 7;4(11):1459–1473. doi: 10.1038/s42255-022-00667-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — a-b, Energy expenditure (EE) of Ckb^fl/fl and Ckb^AdipoqCre male mice (n = 8 per group) reared at 22 °C and then housed for 5 days at 30 °C prior to administration of (a) saline (Sal) or (b) noradrenaline (NA, 1 mg kg⁻¹). c-f, Basal oxygen consumption rates (related to experiments in Fig. 2b–g, j, k) of freshly isolated Ckb^fl/fl and Ckb^AdipoqCre brown adipocytes, treated with (c) PBZ (n = 5 per group), (d) PZS (n = 4 Ckb^fl/fl; n = 3 Ckb^AdipoqCre), (e) RS-17053 (n = 5 per group), or (f) SBI-425 (Ckb^fl/fl: n = 5 per group; Ckb^AdipoqCre: n = 4 per group). g-h, Glycerol release from freshly isolated (g) Ckb^fl/fl and Ckb^AdipoqCre brown adipocytes treated with PBZ compared to vehicle (n = 3 per group) or (h) brown adipocytes treated with SBI-425 compared to vehicle (n = 3 per group). Data are presented as mean ± s.e.m. and n numbers are of biologically independent experiments. a, b, Two-way ANOVA (Fisher’s LSD) from minutes 0 to 21; c-g, Two-way ANOVA (Fisher’s LSD); h, One-way ANOVA (Fisher’s LSD).

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