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. 2022 Nov 7;4(11):1459–1473. doi: 10.1038/s42255-022-00667-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a-b, RT-qPCR analysis of BAT from Adrb1^-/+ and Adrb1^-/- female mice reared at 22 °C, housed at 30 °C for 5 days and then subjected to 30 °C or 6 °C at ZT4 for 24 hours (30 °C: n = 4 per genotype) (6 °C: n = 3 Adrb1^-/+, n = 5 Adrb1^-/-). c, Western blot from BAT of mice treated as in Extended Data Fig. 3a, b (30 °C: n = 3 per genotype) (6 °C: n = 3 Adrb1^-/+, n = 4 Adrb1^-/-). d, Quantification of western blots from Extended Data Fig. 3c (30 °C: n = 3 per genotype) (6 °C: n = 3 Adrb1^-/+, n = 4 Adrb1^-/-). e, Western blot from BAT of wild-type male mice (C57BL6/N, 6–8 weeks of age), reared at 22 °C, housed at 30 °C for 5 days and then subjected to 30 °C or 6 °C at ZT4. 1 hour prior to onset of 6 °C exposure (ZT3), mice were injected i.p. with propranolol hydrochloride (5 mg kg⁻¹ or 10 mg kg⁻¹) or saline (Sal). BAT was harvested 24 hours following 6 °C exposure (n = 2 per group). f, Quantification of western blots from Extended Data Fig. 3e (n = 2 per group). g, Pearson correlation of CKB with ADRA1A mRNA in human BAT (n = 73), third cohort. Red data point indicates outlier based on ROUT method (Q = 0.1%). h, Pearson correlation with outlier identified in Extended Data Fig. 3g removed. i, Pearson correlation of CKB with ADRA1A mRNA in human BAT (supraclavicular adipose tissue) (n = 23), second cohort. j, k, Pearson correlation of CKB with ADRA1A mRNA in (j) human SAT (superficial subcutaneous adipose tissue) (n = 10), first cohort and (k) human SAT (superficial neck adipose tissue) (n = 73), third cohort. l-n, Pearson correlation of CKB with (l) ADRB1, (m) PTGER1 and (n) CXCR7 mRNA in human BAT (adipose tissue proximal to carotid sheath) (n = 10), first cohort. o, Pearson correlation of CKB with ADRB1 mRNA in human BAT (supraclavicular adipose tissue) (n = 23), second cohort. p, Pearson correlation of CKB with ADRB1 mRNA in human BAT (n = 73), third cohort. Red data point indicates outlier based on ROUT method (Q = 0.1%). q, Pearson correlation with outlier identified in Extended Data Fig. 3p removed. Data are presented as mean ± s.e.m. and n numbers are of biologically independent experiments. a, b, d, f, Two-way ANOVA (Fisher’s LSD); g-q, Pearson correlation (two-sided).

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