Extended Data Fig. 4. Knockdown of NPFR in the fat body leads to increased food intake after starvation, reduced starvation resistance, and lower glycogen stores.
(a) Food intake measured by dye assay (medium containing 9% sugar and 8% yeast); n = 8 Cg > , n = 10 Cg > NPFRiTRiP. (b) Survival during starvation (n = 100 Cg > , n = 50 Cg > NPFRiTRiP). (c) Glycogen levels in fed and 15-hour-starved females; all n = 10. All animals were mated females. Bars represent mean±SEM. ns, non-significant. a, c: Two-tailed unpaired Student’s t test. b: Gehan-Breslow-Wilcoxon test.