Extended Data Fig. 5. Knockdown of NPFR in the AKH-producing cells leads to increased preference for and intake of dietary sugar.
(a) Consumption preference using two-choice dye assay; n = 19 DILP2 > , n = 20 DILP2 > NPFRiTRiP. (b) Consumption of 10% sucrose measured by CAFÉ assay; n = 15 AKH > , n = 16 AKH > NPFRiTRiP. (c) Consumption preference for 1% vs. 10% sucrose solution measured by CAFÉ assay; n = 18 NPFRiTRiP/+, n = 16 AKH > NPFRiTRiP. (d,e) Consumption of 10% sucrose measured over 6 hours and 24 hours by CAFÉ assay; n = 18 NPFRiTRiP/+, n = 16 AKH > NPFRiTRiP. (f) Consumption preference using two-choice dye assay; n = 15 AKH > , n = 17 AKH > NPFRiTRiP. All animals were mated females. Bars represent mean±SEM. Box plots indicate minimum, 25th percentile, median, 75th percentile, and maximum values. ns, non-significant. a, d, e, f: Two-tailed unpaired Mann-Whitney U test. b, c: Two-tailed unpaired Student’s t test.